About
I am a 4th year BS-MS research undergraduate student at the Indian Institute of Science Education and Research Kolkata (IISER Kolkata), Majoring in Biological Sciences with a Minor in Chemical Sciences. I’m looking for opportunities to work in pressing topics of biology and biology-chemistry interface in order to gain more practical experiences
My Details
- Birthday: 24 October 2000
- Phone: +91 8159085036
- Adress: Berhampore court, Murshidabad, West Bengal, India, PIN: 742101
- Age: 22
- Degree: BS-MS Dual Degree (by Research)
- Email: ag19ms108@iiserkol.ac.in
I am a person who is energetic, self-motivated, trustworthy, responsible, and hardworking. I am an experienced team player who can adjust to any situation. I can operate effectively in a team setting as well as on my own initiative. I can operate effectively under stress and meet tight deadlines.
Facts
A brief overview of my current academic career:
Projects
Lab Rotations
Publications
Skills
WET LAB TECHNIQUES
- Cell Growth and Expression: Competent Cell Preparation | Transformation | Mammalian Tissue culture and maintenance | Cell Revival | Cell Fractionation | Transfection
- Chromatin Biology: Histone and Mononucleosome isolation | Immunoprecipitation | Chromatin Immunoprecipitation (ChIP)
- Protein Expression and Purification: cDNA Preparation | qRT-PCR | Ni-NTA Pull Down Assay | Fast Protein Liquid Chromatography (FPLC)
- Chromatin Biology: Histone and Mononucleosome isolation | Immunoprecipitation | Chromatin Immunoprecipitation (ChIP)
- Molecular Biology and Biochemistry: PCR | Western Blot | Peptide pulldown | RNA Isolation | Protein Isolation
- Optical Microscopy Total Internal Reflection Fluorescence (TIRF) Microscopy | Epifluorescence Microscopy
COMPUTATIONAL SKILLS
- Programming: Python (NumPy; Jupyter Notebook) | MATLAB
- Bioinformatics: AutoDock | Gromacs | VMD | NAMD | PyRx | Discovery Studio
- Image Analysis: ImageJ
- Data Analysis: Gnuplot | Origin | GraphPad Prism
- Software: LATEX | Gaussian
- Elementary ChIP Seq data analysis: Beginner: Peak-Calling | GO analysis | Venn-Diagram | Pathway Analysis | String Analysis
LANGUAGES
- Native: Bengali
- Fluent: English, Hindi
- Beginner: German
Resume
Sumary
Aritra Ghosh
I am a 4th year undergrad majoring in Biological sciences with a special interest in understanding epigenetic regulations of cell during development and stress.
- West Bengal, India
- +91 8159085036
- ag19ms108@iiserkol.ac.in / aritraghosh.iiserkol@gmail.com
Education
BS-MS Integrated (Research)
2019 - 2024
Indian Institute of Science Education And Research (IISER), Kolkata, India
- Currently in 4th Year
- Department of Biological Science
- Current CGPA : 8.92 out of 10
- Relevant Coursework: Biochemistry | Inorganic Chemistry | Biophysics | Immunology | Cell Biology | Microbiology | Fundamentals of Spectroscopy | Reaction Mechanisms in Organic Chemistry | Molecular Genetics | Structural Biology | Cloning and Protein Expression Laboratory | Biostatistics | Developmental Biology | Protein Engineering | Physiology | Epigenetics.
- Independent Study: Cancer Biology | Cancer-epigenetics | Developmental Biology | Genetic Circuit-making and engineering | Synthetic Biology | Myosin Biology
Research Projects
Regulation of p53 protein upon extreme hyperthermia
SUMMER RESEARCH STUDENT UNDER SUPERVISION OF DR. CHANDRIMA DAS
June-July 2022
SAHA INSTITUTE OF NUCLEAR PHYSICS
- I studied how different stress responses act as master key to epigenomic changes in transcriptome and metabolome for Cancer Etiology
- I contributed in writing a book chapter on action of lncRNA in drug resistance. (To Be Published)
- I hypothesised that global H3K9Me2 mark is vital for nuclear import of necessary proteins upon any stress induction. This hypothesis remains to be tested.
- I participated in a study that showed SIRT2 nuclear import upon 47°C hyperthermia treatment in HepG2 cells.
- My research project included:
- Studying p53 expression levels upon extreme hyperthermia (47°C)
- SIRT1-mediated H4K16ac deacetylation at 47°C.
- p53 nuclear localization upon 47°C hyperthermia.
- p53 ubiquitination status and signalling activation of autophagy mediated cell survival instead of cell death pathway.
- All study was performed on HepG2 cell line
VEGF: An Angiogenic Switch in Ovarian Cancer
SUMMER RESEARCH STUDENT UNDER SUPERVISION OF DR. JAYASRI DAS SHARMA
June - July 2021
INDIAN INSTITUTE OF SCIENCE EDUCATION AND RESEARCH KOLKATA
- Understanding of Fundamentals of Cancer and Cancer Pathogenesis
- Understanding of Basics of Angiogenesis and VEGF Receptor.
- Understanding of VEGF Signaling pathways like:
- VEGF and FLK-1/KDR Signaling Pathway
- VEGF pathway and the AKT/mTOR/p70S6K1 signalling pathway in human epithelial ovarian cancer
- Synergistic effect of VEGF and SEMA4D on promotion of angiogenesis in epithelial ovarian cancer
- AEG1 contribution in metastasis in hypoxia related ovarian cancer by modulating HIF-1 alpha/ NF-kB VEGF pathway.
Laboratory Rotations
Cellular Biophysics
May 2022
SUPERVISED BY PROF. BIDISHA SINHA, IISER KOlKATA
- Learned Total Internal Reflection Fluroscence (TIRF) Microscopy
- Learned Epifluorescence Microscopy
- Learned Phalloidin Staining of Actin
- Learned Optical Twizzers and Optical Trap along with Micro-pattering of HeLa Cells
Histone Epigenetics and Gene Regulation
Dec 2021 - April 2022
SUPERVISED BY PROF. BABU SUDHAMALLA, IISER KOLKATA
- Learned insights of molecular mechanism of histone code recognition by different members of Bromodomain family
- Learned several wet lab techniques involved in protein expression and cell culture.
- Learned Engineering an acetyllysine reader with a photocrosslinking amino acid for interactome profiling.
Theoretical and Computational Biophysics
June 2021 - Nov 2021
SUPERVISED BY PROF. NEELANJANA SENGUPTA, IISER KOLKATA
- Learned different Biophysical theories and methods used in current research
- Learned Membrane-protein interactions
- Learned how to use GROMACS, VMD and NAMD for Molecular Dynamics (MD) simulation studies
Drosophila Genetics
January - March 2020
SUPERVISED BY PROF. MOHIT PRASAD, IISER KOLKATA
- Identification of different mutations in Drosophila melanogaster.
- Type of genes in Drosophila melanogaster.
- Cross setup for different desired phenotype and genotype.
Academic Achievement
Saha Summer Research Fellowship
- Offered to students interested in starting a research career
- Appointed scholar under Dr. Chandrima Das.
- Among the 17 Selected Scholars, against numerous candidates from different Institute of National Importance (INI’s) around India.
- The fellowship grant amounts to 14,000 INR.
June-July 2022
SAHA INSTITUTE OF NUCLEAR PHYSICS
National Initiative on Undergraduate Sciences Biology (NIUS) Scholar
- Offered to students interested in starting a research career
- Appointed scholar under Dr. Sasi Kumar Menon, Director, Therapeutic Drug Monitoring Lab, Institute for Advanced Research and Training in Interdisciplinary Sciences (IATRIS), Mumbai, India.
- Selected Scholar, against 49 candidates from different Institute of National Importance (INI’s) around India, for an enrichment camp, after two round selection and an interview. The book grant amounts to 10,000 INR.
Oct 2019 - Cancelled due to COVID19 Pandamic
HOMI BHABHA CENTRE FOR SCIENCE EDUCATION, HBCSE-TIFR, INDIA
National Standard Examination in Biology (NSEB)
- Center Topper, Round 1 State 23rd Rank Holder
INDIAN ASSOCIATION OF PHYSICS TEACHERS (IAPT), INDIA
Diploma in German language
ATULYA GHOSH INSTITUTE OF LANGUAGES, KOLKATA, INDIA.